13 alt path - A T P H H 1 How are protons pumped 2 What is the nature of the gradient/energetics 3 How many protons are pumped 4 How is the

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Unformatted text preview: A T P H + H + 1. How are protons pumped 2. What is the nature of the gradient/energetics 3. How many protons are pumped 4. How is the gradient maintained 5. How do the protons return to the matrix 6. How is that return coupled to ATP synthesis 7. How many ATP are produced per electron pair 8. How can the system be inhibited/studied Pump 10 protons/NADH Pump 6 protons/FADH 2 Use 3 protons/ATP Use 1 H + /ATP-ADP-Pi transport Total use is 4 protons/ATP 2.5 ATP/NADH 1.5 ATP/FADH 2 Glycolysis 2ATP 2ATP 2 NADH 5 ATP Pyr-Acetyl CoA 2 NADH 5 ATP Krebs Cycle 6 NADH 15 ATP 2 GTP 2 ATP 2 FADH 2 3 ATP Total 32 ATP Glycolysis 2ATP 2ATP 2 NADH 5 ATP Pyr-Acetyl CoA 2 NADH 5 ATP Krebs Cycle 6 NADH 15 ATP 2 GTP 2 ATP 2 FADH 2 3 ATP Total 32 ATP Less the ATP to move NADH to mitochondrion 1. How are protons pumped 2. What is the nature of the gradient/energetics 3. How many protons are pumped 4. How is the gradient maintained 5. How do the protons return to the matrix 6. How is that return coupled to ATP synthesis 7. How many ATP are produced per electron pair 8. How can the system be inhibited/studied Electron Transport Inhibitors Complex I Complex I Complex IV Electron Transport Inhibitors Complex III Uncoupler-Relax Gradient...
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This note was uploaded on 01/12/2010 for the course MCB 100B taught by Professor Alper/calendar during the Spring '08 term at University of California, Berkeley.

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13 alt path - A T P H H 1 How are protons pumped 2 What is the nature of the gradient/energetics 3 How many protons are pumped 4 How is the

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