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Unformatted text preview: Problem Set, Oxidation/Reduction/Krebhs 1. A USC researcher, distracted by dreams of the Rose Bowl, proposes to increase the yield of energy from glycolysis by engineering cells to reduce pyruvate to lactate with USox, a new compound that has an E ' of -0.64. a. The E ' of pyruvate + 2H + + 2e- is -0.19V. With F = 23 kcal/volt/mol, discuss arguments for and against the researcher's prediction that this particular reaction could be used to generate an additional ATP. b. What other factors would you need to worry about before attempting to engineer the fermenting cell to use USox for all applicable reactions in glycolysis. Explain. (Qualitative answers are sufficient. Do not worry about other pathways.) 2. A biochemist decides to improve an organism that ferments glucose to lactate by deleting the step that converts pyruvate to lactate. He reasons that the new organism would be saving the energy that would otherwise have gone into producing the enzyme for that reaction. The new organism would excrete the pyruvate directly. Comment on his idea. 3. Briefly describe the nature of each of the following terms, indicating their biochemical significance with regard to topics discussed in class to date. a. nicotinamide b. pellagra 4. A student repeats the Westheimer experiment involving deuterated ethanol and NAD. She uses however, an enzyme from an organism isolated from a meteor that was shown to catalyze the same reaction. She focuses on enzyme from an organism isolated from a meteor that was shown to catalyze the same reaction....
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