Lecture 9 ENGR305

Lecture 9 ENGR305 - Lecture 9 ENGR 305 Outline Read Chapter...

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Lecture 9 ENGR 305
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Outline Read Chapter 31 in Berg
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How do you know what is going on inside a cell?
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Ideas DNA Some insight, but liver cells, brain cells, pancreas, fingers, etc… all have the same genome RNA, messenger RNA Much better, idea of what the cell is trying to do at a particular moment
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Examples Red Blood Cells (Erythrocytes) mRNAs? α -globin, β -globin Proteins --ditto! Concept: Cells function according to the proteins that are present The Protein complement is dictated by mRNAs Therefore, control mRNA expression, control the cell mRNA synthesis is called Transcription
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DNA Microarray for mRNA Profiling See animation: genechip
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Figure 1.18 The Biology of Cancer (© Garland Science 2007)
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Transcription
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RNA Polymerase: RNA synthesis, DNA template T7 RNA Polymerase; RNA Pol I, II, III DNA Polymerase: DNA synthesis, DNA template T4 DNA Polymerase; Pol I, II, III, IV more RNA Replicase: RNA synthesis, RNA template QB replicase, others Reverse Transcriptase: DNA synthesis, RNA or DNA template Superscript, HIV RT, Terminology
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Lecture 9 ENGR305 - Lecture 9 ENGR 305 Outline Read Chapter...

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