126 syn refinement answers

126 syn refinement answers - Confocal imaging of dendritic...

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10 points: Questions on the papers by Taha and Stryker, due Tuesday 2/3/09 at the start of class: 1) (3 Points) How did ocular dominance change in the following experiments: A) monocular deprivation (MD) with saline infusion in visual cortex; B) MD with cycloheximid infusion in visual cortex; C) MD with cycloheximid infusion in the LGN. A) OD shift to the opened eye after monocular deprivation, or more response to the opened eye. (1pt) B) No OD shift after monocular deprivation, or no change. (1pt) C) OD shift to the opened eye after monocular deprivation, or more response to the opened eye. (1pt) 2) (4 Points) Was the morphology or the physiology of cortical neurons affected by protein synthesis block in V1? What experiments the authors performed to test this? No. 1.
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Unformatted text preview: Confocal imaging of dendritic spine density. 2. Single unit recording. 3. VEP recording. 4. Intrinsic optical imaging of cortical maps. 3) (3 Point) How was cycloheximid delivered to the visual cortex? Why such method is used? How do authors confirm that protein synthesis is blocked? How much reduction in protein synthesis is achieved in V1 block? • Use osmotic minpumps to infuse cyclohexmid directly into the brain. (1pt) • This method is used to establish a reliable, constant inhibition on protein synthesis. (0.5 pt) • Incorporation of tritium-labeled amino acid leucine into nascent proteins, and measure it by autoradiography (or silver grain counts). (1 pt) • ~50% (45 ± 12%) protein synthesis block is achieved at the midpoint of V1. (0.5pt) ....
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This note was uploaded on 01/15/2010 for the course BIO 31402 taught by Professor Lee during the Spring '09 term at UCSC.

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