biochem100bSolutionSet6-1

biochem100bSolutionSet6-1 - BMB100B Winter 2009 Rubin...

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BMB100B Winter 2009 Rubin Solution Set #6 Due Tuesday, March 10th 10:00 A.M. 1) Choosing from steric, oleic, and linoleic acid as fatty acids, draw the structure of a phosphatidylserine, triglycerol, and a sphingolipid. Name your compounds using common fatty acid names. Of all the structures you could have potentially drawn, identify and name the compounds with the lowest and highest melting points. 2) What is the minimum number of residues an α helix must have to span the hydrophobic
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BMB100B Winter 2009 Rubin portion of a phospholipid bilayer? What is the minimum number of residues a β strand must have if it is inserted at a 45-degree angle to the bilayer plane? Why are most secondary structure elements in proteins longer than this minimum amount? 3) In lecture, f luorescence r ecovery a fter p hotobleaching (FRAP) was described as a method
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BMB100B Winter 2009 Rubin for measuring lateral diffusion rates of lipids in membranes. Briefly describe an experiment in which you use FRAP to a) measure the melting temperature of a membrane The melting temperature is the temperature at which the membrane undergoes a sharp transition from a crystalline-like state to a state in which lateral diffusion is facile. In the FRAP experiment described in lecture, lateral diffusion is observed by photobleaching and timing recovery of fluorescence within the photobleached spot. By repeating this experiment as a function of temperature, one could incrementally lower the temperature and find a point at which lateral diffusion becomes minimal. b) measure catalytic activity of a flipase One first needs a method for measuring transverse diffusion rates. One possibility would be to photobleach one side of a membrane in its entirety and image as a function of time. If the entire surface were photobleached, then recovery would only occur from fluorescent lipids flipping over from the transverse side. Once an assay for measuring transverse diffusion is found, by
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biochem100bSolutionSet6-1 - BMB100B Winter 2009 Rubin...

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