Unformatted text preview: Kim et al mice. • Age of the animals. • Compensatory effect: eg. Simone et al mice show high Nogo-B expression in CNS. (give 1 point to each answer matches above key) 2) (2 points) for Simonen et al: Nogo-A, B and C all comes from the same gene. What common sequence do they share? And how did the authors selectively knock out Nogo-A? All Nogos share a common C-terminal domain of 188 amino acid. The authors replaced parts of nogo exons 2 and 3 and the intron between them with the PGK-neo gene, the replaced sequence is specific to NogoA) 3) (2 Points) for Simonen et al: What happens to Nogo-B and Nogo-C expression in the CNS and PNS of Nogo-A-/- mice? Nogo-B: dramatically upregulated (10-fold) in CNS, no change in PNS. Nogo-C: no change in either CNS or PNS....
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- Spring '09
- Neuroscience, Knockout, Spinal cord injury