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LS17DNAcloning - Lecture 17 DNA CLONNING Allows...

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1 Allows manipulation of a specific fragment of DNA in the test tube Reintroduced into cells and grown in large quantities (cloned) Any gene can be isolated, amplified, sequenced and modified for further study Lecture 17: DNA CLONNING Problems in working with DNA DNA fragments of different genes have the similar shape and similar charge to mass ratio, so one gene cannot be easily distinguished from another like proteins. Genes are all connected in vast DNA molecules, each gene is a very small fraction of the entire genome, so a gene can not be isolated like a protein
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