LS20REVIEW - Office Hours Friday, Dec. 4 th , 2-4 pm...

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Unformatted text preview: Office Hours Friday, Dec. 4 th , 2-4 pm Monday, Dec. 7 th , 11 am 1 pm Tuesday, Dec. 8 th , 10 am to 12 pm 210A or 210C Biomedical Science Research Building (BSRB) Ask your TA for extra office hours LS3 Final EXAM 12/08/09 3:00 TO 6:00 PM The Scheduling office has not given us the room assignments. You should check your email on Friday/Saturday. The Final Exam will cover all materials from lecture 1 to lecture 19 It is estimated that the number of described species on earth Is approximately 1.75 million (total may up to 100 million). Central Dogma of Molecular Biology DNA RNA Protein (Gene Expression) Transcription Translation protein sequence comparison G LS DG E WQL V LN V W GKV E A DIPGH G QE V L I R L F KGH P ETLEKF G LS DG E WQL V LN V W GKV E A DLAGH G QD V L I R L F KGH P ETLEKF E LS EA E RKA V QA M W ARL Y A NCEDV G VA I L V R F F VNF P S----- D.M. H.M. H.H D.M. Dolphin myoglobin, H.M. human myoglobin, H.H. human hemoglobin Protein homology : means either identical amino acid or similar amino acid. This is because change of an amino acid in a protein to a different amino acid with similar chemical property usually cause no harm to the protein with respect to its function. Similar amino acids:- acidic: E, D- basic: K, R, (H),- polar uncharged: S, T, N, Q, C, (H), (Y)- hydrophobic: L, I, A, V, G, M, F, P, W, (Y)- some are less sure than others (e.g. Y, H). Proteins are polypeptide polymers made up of amino acids 20 amino acids are used to make up proteins in all organisms on earth. The 3D shape of protein is determined by its amino acid sequence The unlimited 3D shapes (called conformation) that proteins can assume allows them to perform a wide range of functions 2. Protein Structure Lecture 3: Function of proteins-most work in the cell are done by proteins, how do proteins work? 1. Protein family Why different proteins have different functions? 2. How do proteins work?-antibodies -enzymes 3. How are proteins regulated in the cell Lecture 4:Protein analysis-how do we study proteins? I. Separation How to separate different cellular components? and different proteins? (centrifugation, chromatography, SDS-PAGE, etc) II. Detection (immunological techniques) How to detect a single protein from a mixture of proteins (ELISA, Immunoblot, Immunoprecipitation, etc) III. Sequence and Structural analysis Mass spectrometry, X-ray crystallography, etc Comparison between RNA and DNA DNA RNA sugar Deoxyribose ( 2 -H ) Ribose ( 2 OH ) base A, C, G, T A, C, G, U structure double strand single strand size large (kb-Mb) Small (bp-kb) type DNA mRNA, tRNA, rRNA, snRNA Stability in alkaline Stable (ds DNA denatured at high pH but it is not hydrolyzed) Unstable (Hydrolyzed to nucleotides) DNA Double Helix DNA Double Helix Double helix Double helix Right handed Right handed Anti-parallel strands Anti-parallel strands Strands are held together Strands are held together by hydrogen bonding by hydrogen bonding between base pairs on...
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This note was uploaded on 01/16/2010 for the course LIFESCI Life scien taught by Professor Lorenz,t.c. during the Fall '09 term at UCLA.

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LS20REVIEW - Office Hours Friday, Dec. 4 th , 2-4 pm...

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