{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

11_DNA%20analysis

11_DNA%20analysis - Thermodynamic Analysis of...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
1 adenine (A) guanine (G) thymine (T) cytosine (C) Four different bases: DNA adenine (A) guanine (G) uracil (U) cytosine (C) RNA base pairs: A – T A – U G – C G – C Watson-Crick base pairs Thermodynamic Analysis of DNA/RNA (Determination of T m ) Watson-Crick base pairs in DNA A – T G – C [G] = [C] and [A] = [T] Note : Other base pairs can also be formed, but they are less energetically favorable. Example: G 0 (kcal/mol) C – C -1.97 G – G -4.1 to -5.4 C – G -5.4 to -6.8 Melting and Stability of Double Strand DNA DNA melting = unwinding of the double helix One way to study the stability of DNA is to determine its thermal stability. (In cells, DNA melting is mediated by specific proteins.) • Method to determine T m : [DNA] measurement via UV absorption at 260 nm 260 wavelength Absorbance single stranded DNA double stranded DNA The thermal stability is characterized by the melting temperature, T m .
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon