1adenine (A)guanine (G)thymine (T)cytosine (C)Four different bases: DNAadenine (A)guanine (G)uracil (U)cytosine (C)RNAbase pairs:A – T A – UG – C G – C Watson-Crick base pairsThermodynamic Analysis of DNA/RNA (Determination of Tm)Watson-Crick base pairs in DNAA – T G – C[G] = [C] and [A] = [T]Note: Other base pairs can also be formed, but they are less energetically favorable. Example: G0(kcal/mol)C – C-1.97G – G-4.1 to -5.4C – G-5.4 to -6.8Melting and Stability of Double Strand DNA•DNA melting = unwinding of the double helix•One way to study the stability of DNA is to determine its thermal stability. (In cells, DNA melting is mediated by specific proteins.)• Method to determine Tm: [DNA] measurement via UV absorption at 260 nm260wavelengthAbsorbancesingle stranded DNAdouble stranded DNA•The thermal stability is characterized by the melting temperature, Tm.
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