1Binding of small to large molecules• Receptor / ligand binding• Binding of O2to hemoglobin• Binding of carcinogens to DNA• Binding of proteins to DNA• Binding of ions to polymer fibersExamples:A + P krkfPA At equilibrium,kf[A][P] = kr[PA]or]P][APA[kkKrf1. The large molecule has only one binding site.A: small molecule, P: polymer or large moleculeDefine0]PA[bound(average number of binding sites on P that are occupied by A)For the reaction with only one binding site on P,]PAPPAPA[bound0When = 0.5, [A] = 1/K.At equilibrium, ]A[K]A[K]P][A[K]PP][A[K]PAPPA[1depends on T, P, and [A].The curve of versus [A] is called binding isotherm.[A]T
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