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Unformatted text preview: gene circuit (with and without IPTG added) are available at your station. 3. Make two slides from each culture: s Label six microscope slides s Add 25 μ l culture to the middle of microscope slide 4. For one slide of each culture (three slides total), immediately add a coverslip 5. For the remaining three slides: s Place slides on slide holder over sink s Add enough crystal violet stain to cover each slide s Wait 20-60 seconds s Rinse with distilled water using squirt bottle until solution runs clear s Blot with bibulous paper 6. You will view your slides under brightfield and fluorescence microscopy. Record your observations below. Competent cells only GFP gene circuit GFP gene circuit + IPTG Colonies on plate Cells under microscope...
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This note was uploaded on 01/17/2010 for the course BME 100 taught by Professor Yuan during the Fall '07 term at Duke.
- Fall '07