BME%20100%20Lab%202%20Fall%2009

BME%20100%20Lab%202%20Fall%2009 - MEASUREMENT OF BACTERIAL...

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1 MEASUREMENT OF BACTERIAL GROWTH UNDER DIFFERENT NUTRIENT CONDITIONS Safety: The Escherichia coli bacteria used in this lab have been modified to be non-virulent, but may induce an acute immune response in a very small percentage of individuals (this may occur whenever one is exposed to foreign materials or organisms). You must take the following safety precautions: Wash your hands when entering and before exiting the lab Wear a lab coat and gloves (dispose of after the lab) No eating or drinking in the lab Wear closed toe shoes Objectives: In this lab, you will: Analyze growth of bacteria under varying nutrient conditions Background: Growth Phases of Bacteria When normal bacteria are introduced in a fixed resource environment in the wild or in the laboratory (i.e., in growth media with suitable conditions such as temperature, pH, oxygen, and nutrients), cells grow in four phases: lag, log, stationary, and death. 1. Lag phase. Cells grow relatively slowly as they acclimate to the growth conditions and available resources. 2. Log (logarithmic) phase. While resources are plentiful, population density rises exponentially with time. The cycle continues so that a single parent cell is the progenitor of an exponentially increasing number of daughter cells: 2, 4, 8, 16, 32, 64, etc. In E. coli, each cell cycle takes approximately 20-60 minutes. 3.
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This note was uploaded on 01/17/2010 for the course BME 100 taught by Professor Yuan during the Fall '07 term at Duke.

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BME%20100%20Lab%202%20Fall%2009 - MEASUREMENT OF BACTERIAL...

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