BIS104 Slide2 - Lecture 2-Model systems(FYI-How cell...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 2 -Model systems (FYI) -How cell function is studied Prokaryotic Model systems Viruses DNA, RNA & Protein synthesis Gene regulation Control of Cell proliferation & Cancer Infection & Immunity Possible gene therapy tools Bacteria DNA, RNA & Protein synthesis Gene regulation Targets for new antibiotics Cell cycle & signaling Figure 1-29 Molecular Biology of the Cel , Fifth Edition (© Garland Science 2008) Eukaryotic Budding yeast S. cerevisiae Gene regulation Control of cell cycle and cell division Protein secretion Chromosome structure Figure 1-42a Molecular Biology of the Cel , Fifth Edition (© Garland Science 2008)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 1-47 Molecular Biology of the Cel , Fifth Edition (© Garland Science 2008) Round worm C. elegans Development of body plan Cell proliferation Cell lineage & programmed cell death Behavior Fruit fly Drosophila melanogaster Development of body plan Differentiation & development Programmed cell death Behavior Figure 1-48 Molecular Biology of the Cel , Fifth Edition (© Garland Science 2008) Mice: Mus musculus Development of body tissues, physiology Function of the immune system Model for Cancer & other human diseases Both have mutations in the kit gene Figure 1-53 Molecular Biology of the Cel , Fifth Edition (© Garland Science 2008) Plant ( A. thaliana ) Gene regulation Infectious diseases Agriculture applications Physiology How cell function is studied part I - Cell study by cell Culture Some cells can be grown in culture with appropriate media ( sugars, salts, amino acids vitamins ) Bacteria were the first organism for which defined culture conditions were achieved Animal and plant cells require more complex culture Conditions, ex. need growth factors Primary cell culture: most short-lived, secondary culture OK Cell lines: most able to divide indefinitely if culture properly A few facts about cell cultures
Background image of page 2
Examples of cells in culture fibroblasts myoblasts Show cell fusion to form multinucleated fibers Manipulating proteins, DNA and RNA Cell fractionation aids to isolate organelles and macromolecules for biochemical/molecular analysis. Disrupting the cells Cell and macromolecule fractionation 20,000 X g 100,000 x g 1,000 X g RPM : revolutions per minute RCF: relative cent rifugal force, expressed in units of gravity (x g) RCF = g = (1.118 x 10 -5 ) R S 2 R: radius of rotor (cm) S: Speed (RPM) (size & shape based ) Fig. 8-10 Separate single molecules by Figure 8-12 Molecular Biology of the Cel
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/18/2010 for the course BIO SCI 104 taught by Professor Lin during the Spring '09 term at UC Davis.

Page1 / 11

BIS104 Slide2 - Lecture 2-Model systems(FYI-How cell...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online