Lec4_Jan-13-2010_Shreier-Relatedness

Lec4_Jan-13-2010_Shreier-Relatedness - Announcements...

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Announcements Session 4 Jan 13 2010 Make sure HW-1 and HW-2 are handed in by tonight to avoid late penalty on grade. From now on, late penalty if after the due date/time. Updates on Class schedule made every week – check regularly on SmartSite “Syllabus” on left menu – “Class Schedule” file No Class on Monday – HOLIDAY! Read Ch 5 for next class on Wed Jan 20 HW-3 will be posted by 5pm this Fri HW-1 key will be up by 5pm Fri HW-1 and 2 graded and returned; HW-2 Key available by next Tues
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Kinship, Parentage and Relatedness Analyses in Ecology Session 4, 1/13/09 ECL242/PHR242 Andrea Shreier
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Take Home Points Importance of molecular techniques in behavioral ecology Distinction between parentage and kinship/relatedness There are different ways to determine parentage and relatedness Both parentage and relatedness analyses have “pitfalls”
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Kinship Kinship refers to familial relationships between individuals What ecological phenomena can knowledge of kinship help us understand?
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Kinship Kinship refers to familial relationships between individuals What ecological phenomena can knowledge of kinship help us understand? Mating system Reproductive success Altruistic behavior Selection Dispersal
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Why Molecular Analyses Matings difficult to observe Plants Animals Fossorial, arboreal, aquatic Nocturnal Polygamous – who’s the daddy? Difficult to determine family relationships other than parentage by visual observation http://www.freedigitalphotos.net/images/view_photog.php?photogid=587
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Molecular Markers in Kinship Microsatellites (SSR) Tandem repeat of 2 – 4 base pairs SNP (Single Nucleotide Polymorphism) Different nucleotides at a single position AFLP (Amplified Fragment Length Polymorphism) PCR isolated polymorphic DNA Old techniques – Minisatellites, RAPDs
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Pedigrees Often used in Species Survival Plans (SSPs) Not always practical for wild populations Exception : long-term datasets
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Reconstruction of Family Relationships Parentage analysis Collect data from potential parents and offspring Genetic methods assign offspring to parents Relatedness analysis Genetic methods determine relationship between individuals Parent/offspring, full-sibling, half-sibling, uncle, etc.
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Parentage Analysis Uses multiple loci to assign offspring to true parent Microsatellites SNPs See Jones and Ardren (2002) for excellent review of this topic
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Parentage Analysis: Exclusion Simplest form of parentage analysis Uses incompatibilities between offspring and parents to assign parentage Mom Baby Dad Pitfalls – Null alleles Mutation Genotyping error
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Lec4_Jan-13-2010_Shreier-Relatedness - Announcements...

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