{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Note4 - IV Polymerase chain reaction(PCR – review on your...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 4 MCB 161 S. Harmer 01/14/10 Molecular Methods (vectors, libraries) DNA replication (part I) Reading: Watson text: pp 25 - 28; 120 - 126; 195 - 209; 230 – 254; plus, watch the animations posted on the ‘Assigned Reading’ web page. Problems: MBOC problem book: 5-20, 5-39, 5-41, 5-49, 5-53, 5-58 (A and B only), and 5-60 Molecular Methods I. Directional cloning II. in vitro transcription and translation; expression vectors III. Libraries A. Genomic libraries B. Screening libraries with nucleic acid probes
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: IV. Polymerase chain reaction (PCR) – review on your own Overview of DNA replication I. Chromosome features A. Centromeres – essential for proper segregation during mitosis and meiosis B. Telomeres – protect ends of chromosomes, ensure complete replication II. General replication mechanisms A. Semi-conservative and semi-discontinuous B. Leading and lagging strand synthesis primed by RNA primers C. Distinct origins of replication...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online