Old exam#1with answer

Old exam#1with answer - Bio Sci 104 Winter 2009 MIDTERM #1...

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Bio Sci 104 Winter 2009 MIDTERM #1 (Lecture 2-9) A. There are three versions of the exam with the same questions but in different order. B. All questions have equal value. 40 questions total (3 points each). C. Number of * denotes the number of correct answers (questions 1-35). You need to mark ALL correct answers to get ANY credit . No penalties and no partial credits . D. It is essential that you fill out the following both on this sheet and on Scantron (bubble in your student ID). Failure to do so may result in loss of some or all credit for this exam. Your name _____________________________________________________________ Your student ID _________________________________________________________ The version of this exam: ________ a _____________________________ *1. Pretending you were a scientist working for the “Jurassic park” biotech company. One day, you discovered a piece of fossil in a field trip. You suspected that it might belong to a particular species of dinosaur. In order to decode the DNA sequences, you have to first amplify the DNA of a few highly conserved genes from the fossil. Which of the following technique allows you to complete this task? a. microarray analysis b. polymerase chain reaction c. fractionation by centrifugation d. in situ hybridization e. using the pulse-chase method with radioactive nucleotides. **2. After analyzing the DNA sequences, you found that one gene “DIN” showed ~90% identity to a gene “BIR” that was found in many birds. What are the appropriate terms for describing the relationship of these two genes? a. homologs b. paralogs c. catalogs d. orthologs e. dialogs *3. Now you suspect that dinosaurs are actually giant birds (without feathers). In order to gain further insight into the function of the protein encoded by DIN, you cloned this gene into a protein expression vector and made a lot of DIN protein in an E coli host. Before you purify the DIN protein, you happened to know that the BIR protein has been widely studied and antibodies recognizing the BIR protein are commercially available. Which type of analysis would help you determine whether the anti-BIR antibodies would also recognize the DIN protein? a. Southern blot analysis b. Western blot analysis c. Northern blot analysis d. Microarray analysis e. Ion-exchange chromatography
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Bio Sci 104 Winter 2009 *4. Assuming that the Anti-BIR antibody was able to recognize the DIN protein. Based on the information provided here, which of the following chromatography methods is the best way to purify the DIN protein from the cell extract of the E coli host? a. ion-exchange chromatography using positively charged beads b. ion-exchange chromatography using negatively charged beads c. gel-filtration chromatography using porous beads d. affinity chromatography using beads coated with the BIR protein e. affinity chromatography using beads coated with the anti-BIR antibody ***5. Which of the following statements are correct? a. microarray analyses allow amplification of specific genes.
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Old exam#1with answer - Bio Sci 104 Winter 2009 MIDTERM #1...

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