Chapter 2.3 - 2.4 - 15 2. KINETICS AND STOICHIOMETRY OF...

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Unformatted text preview: 15 2. KINETICS AND STOICHIOMETRY OF CELLULAR GROWTH BE 421 2. KINETICS AND STOICHIOMETRY OF CELLULAR GROWTH 2.1. Introduction: review of exponential growth 2.2. Substrate limited growth 2.3. Growth stoichiometry 2.4. Kinetic and stoichiometry of bioproduct synthesis 2.5. Prediction of stoichiometries based on black box models 2.6. The respiratory quotient 2.7. More complex situations: Crabtree effect and limited respiratory capacity 16 Y X/S = " " = ! X ! S r X r S 2.3.1 Growth limited by the C&E Substrate Stoichiometry very important: Indicates how much biomass or product is obtained from one unit of resources, i.e. one g or mole of carbon and energy substrate: S + Y O/S O 2 + Y N/S NH 4 + + …. => Y X/S X + Y P/S P + ….. t s x x s x x s s Y X/S Y X/S = " " = ! X ! S r X r S 17 Strain Substrate ! Yx/s Y'x/s- g/g C-mol/C-mol Candida lipolytica Citrate 3.0 0.290 0.359 Candida lipolytica Succinate 3.5 0.344 0.392 Kluyveromyces fragilis Glucose 5 g/l 4.0 0.520 0.605 Kluyveromyces fragilis Glucose 15 g/l 4.0 0.465 0.540 Kluyveromyces fragilis Glucose 40 g/l 4.0 0.388 0.451 Candida pseudotropicalis Glucose 4.0 0.465 0.539 Candida utilis Glucose 4.0 0.481 0.558 Escherichia coli Glucose 4.0 0.416 0.482 Enterobacter cloacae Glucose 4.0 0.345 0.399 Kluyveromyces fragilis Galactose 4.0 0.494 0.557 Kluyveromyces fragilis Lactose 4.0 0.485 0.5100....
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Chapter 2.3 - 2.4 - 15 2. KINETICS AND STOICHIOMETRY OF...

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