Chapter 2.1 - 2.2 - BE 421 2. KINETICS AND STOICHIOMETRY OF...

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1 2. KINETICS AND STOICHIOMETRY OF CELLULAR GROWTH BE 421 2. KINETICS AND STOICHIOMETRY OF CELLULAR GROWTH 2.1. Introduction: review of exponential growth 2.2. Substrate limited growth 2.3. Growth stoichiometry 2.4. Kinetic and stoichiometry of bioproduct synthesis 2.5. Prediction of stoichiometries based on black box models 2.6. The respiratory quotient 2.7. More complex situations: Crabtree effect and limited respiratory capacity 2 1. Cell counting 3. Counting CFU (viability) 2. Turbidometry see following page 4. Counting by Coulter Counter 5. Gravimetry Determination of dry biomass and its concentration in g/l Référence: J.E. Bailey and D.F. Ollis, Biochemical Engineering Fundamentals, McGraw-Hill, N.Y., 1977. Methods for monitoring and measuring growth
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3 I 0 I OD = 10 log(I 0 / I) Référence: J.E. Bailey and D.F. Ollis, Biochemical Engineering Fundamentals, McGraw-Hill, N.Y., 1977. Measuring growth by turbidometry 4 Cells multiply by doubling their numbers after a certain amount of time, t D . This gives rise to geometrical progressions: 1 - 2 - 4 - 8 - 16…. The simplest law is thus
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This note was uploaded on 01/19/2010 for the course FSH BE223 taught by Professor Ursvonstockar during the Spring '08 term at Dublin City University.

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Chapter 2.1 - 2.2 - BE 421 2. KINETICS AND STOICHIOMETRY OF...

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