Chapter 1.1 - BE421 BIOREACTION ENGINEERING: Enzyme and...

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1 Urs von Stockar Michal Dabros BIOREACTION ENGINEERING: Enzyme and Microbial Kinetics BIOREACTION ENGINEERING: Enzyme and Microbial Kinetics BE421 2 OBJECTIVES OBJECTIVES ! Modelling and design of bioreactors ! Selection of bioreactor type ! Specification of instrumentation BE 421: Bioreaction Engineering PREREQUISITES: Fundamentals PREREQUISITES: Fundamentals ! Understand enzyme kinetics ! Understand kinetics and stoichiometry of microbial growth and product formation " Part on Enzyme and Microbial Kinetics!!
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3 1. ENZYME KINETICS BE 421 1. ENZYME KINETICS 1.0. The role of kinetics in biotechnology 1.1. Enzymes: what they're used for, what they are, and how they work 1.2. Michaelis-Menten kinetics 1.3. Enzyme inhibition 1.4. The influence of pH 1.5. The influence of temperature 4 BE 421: Bioreaction Engineering Cultures at the scale of: the laboratory… …a pilot plant… …giant industrial scale
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5 Type of culture Catabolic metabolites 10 - 20 h 10 - 50 g/l 0.5 - 5 g/(l*h) Bioconversions 10 - 20 h 1 - 10 g/l 0.05 - 0.5 g/(l*h) Antibiotics 48 - 72 h 10 - 50 g/l 0.01 - 1 g/(l*h) Recombinant proteins 3 - 5 jours 5 - 100 mg/l 1 - 50 mg/(l*d) Productivity Duration Final concentration TYPICAL PRODUCTIVITIES IN BATCH
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Chapter 1.1 - BE421 BIOREACTION ENGINEERING: Enzyme and...

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