Exp 13; Spectrophotometric Determination of Food Dye Lab Report.docx

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Sydney CrewsConnor SteeleJoshua DavisCH 228February 14, 2020Experiment 13: Spectrophotometric Determination of Food DyeIntroductionArtificial colors and flavors in food additives are a very common addition to the American diet nowadays. There is a concern whether or not these products are safe to consume.The purpose of this experiment was to determine the amount of food dye present in popular sports drinks by creating a stock solution and four standard solutions using a given food dye.Part A of the experiment had us prepare a 500-mL stock solution. Using the stock solution, Part B had us prepare four standard solutions of different concentrations and label them A. B, C, and D. Part C of the experiment had us determine λmaxusing the absorption spectra of our food dye solutions. Part D was similar to Part C in that we were to determine λmaxusing the absorption spectra, but we used sports drinks instead of our food dye solutions.For safety in this experiment, we were to treat the food dye as any other chemical. We were not to touch, taste, or inhale the powder, and we were to minimize skin contact with the solution because the dye stains both skin and clothes. We were to wear googles at all times and gloves when handling the dye. In addition we were to be careful with the glassware and not insert any volumetric pipette into volumetric flasks because the pipette could snap and break off inside.The beginning questions we had for this experiment were: What is the concentration of dye in each of the sports drinks that we tested? What is the relationship between C andA?ExperimentalData and Observations Calculating the Molarity of the Stock Solution:0.0144g FDC(¿¿1)(mol792.86g)=1.816×105mol1.816×105mol0.500L=3.63×105MCalculating the Concentration (M) of the Standard Solutions:M1V1=M2V2M2=M1V1V2
MA=(3.63×105M)(0.002L)(0.1L)=7.27×107MTABLE 1: Data for Absorbance and Concentrations at λmaxfor Standard SolutionsStandard SolutionAbsorbanceConcentration (M)A0.0987.27E-07B0.2331.82E-06C0.4033.63E-06D0.6025.45E-06Observations:

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