PS#2_W10 - a operon b polycistronic c monocistronic d alternative splicing 9 How does lagging strand synthesis in DNA replication differ from leading

PS 2, 1 Problem Set #2 BIMM100 Winter 2010 – Short Answers 1. What is the difference between conservative and semi-conservative DNA replication? 2. Describe the experiment that led to the conclusion that DNA replication is semi-conservative. 3. In general terms describe the three main types of RNA processing that occur in eukaryotes. 4. What are the interesting features of the 5’ cap? When is the cap added? 5. What is a gene? Do genes code only for proteins? 6. Is mRNA complementary to the template or the nontemplate DNA strand? Can a gene be transcribed in both directions from a single strand of DNA? Why or why not? 7. Name the three stages of transcription discussed in class. What is a transcription bubble? 8. Define the following terms and indicate if they are associated with prokaryotes or eukaryotes:

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Unformatted text preview: a) operon b) polycistronic c) monocistronic d) alternative splicing 9. How does lagging strand synthesis in DNA replication differ from leading strand synthesis? What is an Okazaki fragment? 10. What is a replication fork? A replication bubble? 11. Describe the function of each enzyme or protein listed below in DNA replication: a) Topoisomerase b) Helicase c) Primase d) DNA polymerase α e) DNA polymerase δ f) Ribonuclease H + FEN I g) SSBs (RPA) h) clamp loader (Rfc) i) sliding clamp (PCNA) j) DNA ligase 12. Name and describe the three functional elements chromosomes must have for successful replication and stable inheritance....
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