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Ch369_f09_replic_not - DNA replication Chapter 17 Central dogma of molecular biology Review DNA structure Watson-Crick base pairing in DNA

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DNA replication Chapter 17
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Central dogma of molecular biology:
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Review DNA structure. Watson-Crick base pairing in DNA.
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sugar-phosphate backbones
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Major and minor grooves in DNA. minor major major groove minor groove Rotate 30 degrees around this axis.
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When DNA structure was discovered in 1953, it was immediately recognized that DNA could serve as a template for its own replication. The new DNA strands are a hybrid containing one parental DNA strand (blue) and one newly-synthesized strand (red).
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Initially, 3 different models for DNA replication were considered: Semi-conservative ” was shown to be the correct model in 1958, with key experiments being performed by Meselson and Stahl (where density of DNA enriched in 15 N or 14 N nitrogen isotopes was measured before and after replication).
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DNA replication: Bacterial DNA caught in the act.
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DNA is “negatively supercoiled” (unwinds when stretched).
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DNA topoisomerase is an enzyme that can change the amount of supercoiling in DNA: To change supercoiling, topoisomerase breaks and re-joins the DNA strands . The process requires energy (provided by ATP hydrolysis).
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DNA replication in bacteria (which have a single, circular chromosome).
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Enzymes called “ helicases ” convert double-stranded DNA to single-stranded DNA. Energy is provided by ATP.
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This note was uploaded on 01/21/2010 for the course CH 369 taught by Professor Kbrowning during the Fall '07 term at University of Texas at Austin.

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Ch369_f09_replic_not - DNA replication Chapter 17 Central dogma of molecular biology Review DNA structure Watson-Crick base pairing in DNA

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