PS#3_W10 - stable inheritance 10 Describe in general terms the enzymes involved in the restriction modification system found in bacteria Why are

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PS 3, 1 Problem Set #3 BIMM100 Winter 2010 – Short Answers 1. In terms of cloning, what is a transformation? Under what conditions is a transformation performed in the laboratory? 2. Distinguish between inducible and repressible operons and give an example of each. 3. What is the difference between a strong and weak promoter? Why are they strong or weak? 4. Explain how the lac operon has an “on/off” switch and a “volume control”. Describe the mechanisms involved in regulation of this operon. 5. How do restriction enzymes get their names? 6. Draw a fragment of DNA that has been cut at both ends by the enzyme Eco RI. Where are the phosphates and hydroxyl groups located on the cut DNA? 7. Name and describe three important features of plasmids used for recombinant DNA techniques. 8. How is reverse transcription involved in the process of lengthening telomeres? 9. What are the three important features of chromosomes necessary for replication and
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Unformatted text preview: stable inheritance? 10. Describe, in general terms, the enzymes involved in the restriction modification system found in bacteria. Why are both enzymes necessary? 11. What is meant by the “end replication problem”? 12. Name two cell types where you expect to find telomerase enzyme activity. 13. Why is it important for DNA polymerases to have 3’ to 5’ exonuclease activity? 14. What kind of DNA damage does “end-joining” repair? Why is this mechanism error prone? 15. Distinguish between an exonuclease and an endonuclease. 16. What does “relaxed mode of replication” mean? 17. How do bacteria respond to a changing environment? 18. What do sigma factors (ex. σ 70 ) do during transcription initiation in prokaryotes? 19. What is an enhancer? Do enhancers have to be close to the promoter? 20. What does quorum sensing mean? What is an example?...
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This note was uploaded on 01/21/2010 for the course ECON 100A taught by Professor Staff during the Fall '08 term at UCSD.

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