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Topic 7 Talaro7th-Chapters11,12

Topic 7 Talaro7th-Chapters11,12 - Controlling...

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1 Controlling Microorganisms [Chapters 11,12 Talaro] I. Controlling microbes “outside” the body [Chapter 11] A. Levels of microbial resistance to physical/chemical methods 1. Highest resistance 2. Moderate resistance 3. Least resistance B. Microbial control terminology [Table 11.2] 1. Suffixes: -cidal vs. -static 2. Levels of decontamination - Sterilization - Disinfection - Asepsis/Degerming [use of antiseptics] - Sanitization C. Overview of control methods [Fig. 11.1] 1. Physical agents: heat, radiation 2. Chemical agents: gases, liquids 3. Mechanical removal: filtration [not killing microbes, but removing them] D. Selected factors that influence microbial killing [Fig. 11.2] 1. Number of microbes present 2. Nature and physical form of microbe 3. Environmental conditions: Temperature, pH 4. Presence of contaminating organic material E. Modes of action 1. Heat – removes water & breaks chemical bonds, denaturing proteins and destroying their function; incineration burns up (oxidizes) material 2. Radiation - nonionizing radiation is a mutagen
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