Slides4 - to your protein of interest) 3. Purify fusion...

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1/13/10 1 www.neb.com Portion of a bacterial expression vector 1. Gene of interest cloned into MCS, in frame with bacterial malE gene 2. Bacteria make fusion protein (malE fused to your protein of interest) www.neb.com In vitro purification of fusion protein 1. Gene of interest cloned into MCS, in frame with bacterial malE gene ( malE encodes maltose binding protein (MBP)) 2. Bacteria make fusion protein (MBP fused
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Unformatted text preview: to your protein of interest) 3. Purify fusion protein using bacterial portion as a tag. In vitro purification scheme The multi-cloning site (MCS) of pBluescript www.stratagene.com Fig.7-7 Centromeres essential for chromosome segregation 1/13/10 2 Heterochromatin and euchromatin H E http://www.bu.edu/histology/p/20104ooa.htm...
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This note was uploaded on 01/24/2010 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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Slides4 - to your protein of interest) 3. Purify fusion...

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