BIS104 Slide6

BIS104 Slide6 - Lecture 6 1. Protein trafficking -continued...

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Lecture 6 1. Protein trafficking -continued 2. Membrane as information interfaces - membrane potential, gated channel, synapses
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Endocytosis
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Figure 13-42a Molecular Biology of the Cell (© Garland Science 2008) Pinocytosis: fluid and memb Specific macromolecules Vesicles formed on the plasma membrane (inward vesicles)
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Pinocytosis: formation of vesicles from Clathrin coated pits in the plasma membrane Figure 13-48 Molecular Biology of the Cell (© Garland Science 2008)
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Figure 13-53 Molecular Biology of the Cell (© Garland Science 2008) Receptor-mediated endocytosis
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Page 704 Molecular Biology of the Cell (© Garland Science 2008)
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Figure 12-8 Molecular Biology of the Cell (© Garland Science 2008) Transport in and out of the nucleus - gated transport polyribosomes
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Figure 12-9a Molecular Biology of the Cell (© Garland Science 2008) Nuclear pore complex Nucleus membrane proteins - synthesized on nuclear membrane associated ribosomes Nucleoplasm proteins - synthesized in cytosol as soluble peptides then transport to nucleus Components of the nuclear pore complex contain large number of FG repeats to facilitate transport (F: phenylalanine; G: glycine)
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Figure 12-9b Molecular Biology of the Cell (© Garland Science 2008) Figure 12-9c Molecular Biology of the Cell (© Garland Science 2008) View from outside View from inside A side view
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Figure 12-10 Molecular Biology of the Cell (© Garland Science 2008) -Small proteins and molecules (<60,000 MW) can travel passively through nuclear pore and equilibrate between cytoplasm and nucleus Large proteins and molecules require active transport
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Nuclear Targeted protein (NLS) NLS not cleaved (NLS): nucleus localization signals (K, R, P) -a single sequence or scattered patches Transport through nuclear pore complex (NPC) Nuclear import receptor Ran-GTP Nuclear import receptors hop on the FG repeats on the NPC (FG repeats serve as the binding site)
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Figure 12-16b Molecular Biology of the Cell (© Garland Science 2008) Binding of the Ran-GTP cause the import receptor to release their cargo Cytosol Nucleus
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Figure 12-14 Molecular Biology of the Cell (© Garland Science 2008) Compartmentalization and cycling of the Ran-GDP and Ran-GTP
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Figure 12-15 Molecular Biology of the Cell (© Garland Science 2008) How Ran-GTP hydrolysis provides directionality
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Transport of proteins into mitochondria and chloroplasts Figure 12-21 Molecular Biology of the Cell (© Garland Science 2008)
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Figure 12-22 Molecular Biology of the Cell (© Garland Science 2008) Signal sequence for mitochondrial protein import Amphipathic α helix -recognized by specific receptor Signal sequence is cleaved after transport Red: “+’ charged Yellow: nonpolar Blue: polar but not charged
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BIS104 Slide6 - Lecture 6 1. Protein trafficking -continued...

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