Lec5a_Jan-20-2010_Ernest-Microsat

Lec5a_Jan-20-2010_Ernest-Microsat - MICROSATELLITES Todays...

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Today’s Session Molecular Marker highlight: microsatellites Population Genetics part 1 Announcements for Wednesday Jan 20 No homework for next week Homework-3 due date now Fri Jan 22 Quiz Mon Jan 25 covering first 5 sessions through Ch 5 and Jan 20 Lec (not including Term Paper idea paragraph due Jan 27. MICROSATELLITES ECL242 / PHR 242 Ecological Genetics Jan 20, 2010
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Molecular Markers “Markers are polymorphic proteins or DNA sequences that can be used as indicators of genome-wide variation.” SNPs – Single Nucleotide Polymorphisms Sequences of cytoplasmic DNA - mtDNA or cpDNA RFLP - Restriction fragment length polymorphisms AFLP - amplified fragment length polymorphisms RAPD random amplified polymorphic DNA Microsatellites - Short tandem repeats with alleles represented by different numbers of repeats
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Microsatellite Markers • What are microsatellite markers? • How do we detect them? • What can they be effectively used for? • How much does it cost to use them? Thanks to L. Souza for and students of ECL290 several slides
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Microsatellites Other names • Simple Sequence Repeats (SSR) • Variable Number Tandem Repeats (VNTR) • Short Tandem Repeats (STR) • MLVA Multi-locus Variable Number Tandem Repeat (bacteria)
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Microsatellites Found in every organism investigated so far Highly abundant in the eukaryotic genome 100,000 microsatellites in humans Evenly spaced throughout the genome Different organisms have repeat sequences that are more common than others
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This note was uploaded on 01/24/2010 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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Lec5a_Jan-20-2010_Ernest-Microsat - MICROSATELLITES Todays...

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