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hw5 - should increase as ionic strength is lowered Your...

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Massachusetts Institute of Technology Harvard-MIT Division of Health Sciences and Technology and Department of Electrical Engineering and Computer Science HST.410J/6.07J Homework Assignment #5 Issued: March 15, 2007 Due: April 5, 2007 Determine whether volume change of a cell depends on the isotonic volume. In today’s and next Tuesday’s lab you will be trapping individual cells in a microfluidic de- vice. Once the cells are trapped, you can change the perfusates to expose the cells to a variety of solutions. You can then measure the volume change of the cells in each solution. For example, you can dilute PBS with DI water to measure swelling as a function of ionic strength (swelling
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Unformatted text preview: should increase as ionic strength is lowered). Your homework assignment is to compare individ-ual cells to determine whether the volume change in these various solutions depends on the initial (isotonic) volume of the cell. Write a short report (three pages plus supporting images and graphs) to document your findings. Harvard-MIT Division of Health Sciences and Technology HST.410J: Projects in Microscale Engineering for the Life Sciences, Spring 2007 Course Directors: Prof. Dennis Freeman, Prof. Martha Gray, and Prof. Alexander Aranyosi...
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