fluid_reservoirs - Harvard-MIT Division of Health Sciences...

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Fluid Reservoirs One of the key challenges with using microfluidic devices is getting fluids into and out of them. Several techniques have been developed. One common method is to insert plastic tubing that is connected to a motorized pump, but this approach is expensive and can be challenging to master. Here we will show several simple techniques. The first technique is to punch small holes into the device before bonding, and insert pipette tips filled with fluid into these holes. The image below shows a device with five holes punched into it to act as inlets and outlets. To insert a pipette, first a droplet of water is placed over the hole to guarantee that no air bubbles are introduced into the device. Harvard-MIT Division of Health Sciences and Technology HST.410J: Projects in Microscale Engineering for the Life Sciences, Spring 2007 Course Directors: Prof. Dennis Freeman, Prof. Martha Gray, and Prof. Alexander Aranyosi Next, a pipette tip filled with fluid is inserted into the hole. The pipette tip must not contain any air bubbles. One advantage of this technique is simplicity; it is relatively easy
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to punch holes into PDMS, and pipette tips are plentiful. Another advantage is that it is
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This note was uploaded on 01/24/2010 for the course HST. 410J / taught by Professor Alexanderaranyosi during the Spring '07 term at MIT.

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fluid_reservoirs - Harvard-MIT Division of Health Sciences...

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