131106outline9 - Biol. 1311 9/18/06 Transcription ala E....

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Biol. 1311 – 9/18/06 – Transcription ala E. coli Here we are again at the central dogma (Fig 15.10) RNA polymerase starts in a regulated place and goes only one direction (16-1) DNA is only exposed in short patches (no eq. Fig) And there is a defined stopping point What we can learn from bacteria (Fig. 17.1) Multiple ways to have genes turned to proteins, or not Focus your attention on transcription for now A very short course in bacterial metabolism (Fig 17.4) Or feeding E. coli one sugar at a time Lactose is not the favorite diet, but it will do Some details of organization of lactose metabolism genes (Fig 17.12 eq.) Promoter region is the point at which regulation occurs Understand the other names as well The strategy is to keep genes off unless needed (Fig 17.5a) Why make an RNA if there is no lactose? How did RNA polymerase get there in the first place? (no eq. Fig) Promoter sequences at lac genes Consensus is like a vote – the majority wins How the presence of lactose is detected depends on the lacI protein (Fig 17.5 b)
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131106outline9 - Biol. 1311 9/18/06 Transcription ala E....

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