320Fall09Lecture11

320Fall09Lecture11 - BIO 320 Cell Biology, Fall 09 Dr....

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
BIO 320 Cell Biology, Fall ‘09 Dr. Thomas Bushart Lecture 11
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Topics Mitochondria and chloroplast transport I TIMs and TOMs I Hsp proteins I Multiple possible destinations
Background image of page 2
Multiple membranes results in several unique spaces and surfaces within on organelle
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Basic mechanisms Signal sequences One or two translocation complexes (to cross one or both membranes) Outer mitochondrial membrane contains porins I Free transport of ions and small metabolites I Blockage to proteins Inner membrane impermeable to ions I Problem with outer membrane?
Background image of page 4
Targeting sequences Functionally similar to NLSs Common feature is amphipathic helix Always removed after import
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Protein translocators TOM complex (t ranslocase of o uter m embrane) SAM complex (outer membrane) ranslocase of i nner m embrane) OXA complex (inner membrane)
Background image of page 6
TOMs and TIMs
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
State of proteins Unlike nuclear transport, the mitochondrial translocators are are not large enough to move folded proteins Mitochondrial precursor proteins are unfolded polypeptides I An unfolded polypeptide may be problematic due to non-specific aggregation I Hsp70 proteins act as chaperones to stabilize unfolded proteins
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/25/2010 for the course BIO 51055 taught by Professor Bushart during the Fall '09 term at University of Texas.

Page1 / 27

320Fall09Lecture11 - BIO 320 Cell Biology, Fall 09 Dr....

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online