320Fall09Lecture15 (1)

320Fall09Lecture15 (1) - BIO 320 Cell Biology, Fall 09 Dr....

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BIO 320 Cell Biology, Fall ‘09 Dr. Thomas Bushart Lecture 15
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Topics Lysosomes Endosomes Endocytosis/exocytosis
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Lysosomes Highly varied structurally Common features: I Low pH, ~5 I Contain acidic hydrolases Figure 13-37&38 Molecular Biology of the Cell (© Garland Science 2008)
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Lysosomal pH Acid hydrolases only active in acidic environment pH maintained by V- type ATPase pump Break down nearly everything! I Dangerous to the cell? What would happen if a lysosome ruptured? Transport of acid hydrolases? Figure 13-36 Molecular Biology of the Cell (© Garland Science 2008)
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Lysosomal pathways Figure 13-42 Molecular Biology of the Cell (© Garland Science 2008)
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Autophagy Unique problem of degrading an entire organelle due to topology I Solution to topology similar for degrading transmembrane proteins Figure 13-41 Molecular Biology of the Cell (© Garland Science 2008)
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Hydrolase targeting GlcNAc-phoshate is added in cis Golgi and then trimmed in trans to form mannose 6- phosphate (M6P) tags Not to scale! Figure 13-43 Molecular Biology of the Cell (© Garland Science 2008)
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Hydrolase targeting Hydrolases are recognized for tagging by their signal patches Figure 13-45 Molecular Biology of the Cell (© Garland Science 2008)
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Hydrolase protein transport Forward by clathrin, receptor retrieval by retromer (similar to KDEL receptor) Figure 13-44 Molecular Biology of the Cell (© Garland Science 2008)
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Endocytosis Formation of vesicles at PM for uptake of
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This note was uploaded on 01/25/2010 for the course BIO 51055 taught by Professor Bushart during the Fall '09 term at University of Texas.

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320Fall09Lecture15 (1) - BIO 320 Cell Biology, Fall 09 Dr....

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