320Fall09Lecture14 - BIO 320 Cell Biology, Fall 09 Dr....

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BIO 320 Cell Biology, Fall ‘09 Dr. Thomas Bushart Lecture 14
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Topics Endoplasmic reticulum Vesicle trafficking basics ER to Golgi
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Vesicle docking SNARE proteins give specificity and help fusion I V-SNAREs are usually on vesicles I Complimentary t-SNAREs are usually on target membrane
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Trans-SNARE complexes 4 α -helix bundle: 3 t-SNAREs, 1 v-SNARE I At least 1 t-SNARE must be transmembrane Figure 13-16 Molecular Biology of the Cell (© Garland Science 2008)
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Docking vs fusion Docked vesicles can wait for signals before fusing SNAREs in fusion I Stabalizing energy of quadruple helix overcome energy barrier of forcing two membranes together I Maybe not? Diagram should make it obvious why we have to remove protein coat! Figure 13-17 Molecular Biology of the Cell (© Garland Science 2008)
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Envelope virus fusion Viruses lend support for the SNARE-mediated fusion Figure 13-19b Molecular Biology of the Cell (© Garland Science 2008)
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SNARE recycling Chaperone-like ATPase protein NSF unwinds paired SNARE complexes I Adaptor proteins aid in NSF binding to SNAREs Figure 13-18 Molecular Biology of the Cell (© Garland Science 2008)
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Fusion control? SNARE-only vesicles fuse very slowly in vitro M Signals (Ca 2+ for example) induce fusion SNAREs could be kept unavailable until needed by keeping used SNAREs paired I Rabs possible in regulating NSF?
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This note was uploaded on 01/25/2010 for the course BIO 51055 taught by Professor Bushart during the Fall '09 term at University of Texas at Austin.

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320Fall09Lecture14 - BIO 320 Cell Biology, Fall 09 Dr....

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