BIOL 595-8-27-09 (F 2009) - BIOL 595 (Fall 2009) (8-27-09)...

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BIOL 595 (Fall 2009) (8-27-09)
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Lecture Outline 2) Gamatogenesis 3) Fertilization 1) Primordial Germ Cell (PGC) development
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Primordial Germ Cell (PGC) Development
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Development of Primordial Germ Cells (PGCs)
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Migration of Primordial Germ Cells 1) PG C s  – a ris e  fro m  a  g e rm  la ye r b o th diffe re nt in  kind a nd fa r re m o ve d fro m  the  de ve lo ping  g o na d 2) PG C s - a ris e  in the  yo lk s a c  e ndo de rm  a nd the n  m ig ra te  to  the  g e nita l ridg e  o f the  m e s o de rm  a t  the  m idb o dy le ve l 3) No t o nly do  the  PG C s  c ha ng e  fro m  e pithe lia l to   m o tile  c e lls , b ut the y a re  a b le  to  “ho m e ” to  the ir  c o rre c t lo c a tio n o ve r ve ry lo ng  dis ta nc e
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How is PGC migration initiated?
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1)PGCs are the embryonic precursors of gametes 2)PGC s express specific gene markers alkaline phosphatase and vsa (RNA binding factor) 3)Formation of PGCs is induced by Bmp signaling pathway (Bmp4/Bmp8b) 4)The initially formed PGCs are clustered and non-motile 5)Expression of fragillis (homotypic cell-cell adhesion molecule overlaps where PGCs are formed 6)Downregulation of fragillis expression causes PGCs to be scattered and moved onto endoderm
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How do PGCs find their targets?
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C-kit gene Mutant Mice 1) C-kit gene – encodes for a membrane- bound receptor tyrosine kinase (RTK) 2) C-kit gene in mice has a number of alleles, some of which are fatal in the homozygous condition 3) Phenotypes include severe anemia, abnormal skin pigmentation, and no PGCs (sterile)
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This note was uploaded on 01/27/2010 for the course BIOL 595 taught by Professor Staff during the Spring '08 term at Purdue University-West Lafayette.

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BIOL 595-8-27-09 (F 2009) - BIOL 595 (Fall 2009) (8-27-09)...

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