BIOL 595 9-28-09 (F 2009)

BIOL 595 9-28-09 (F 2009) - BIOL 595 (Fall 2009) 9-28-09...

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BIOL 595 (Fall 2009) 9-28-09
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Cell-cell Interaction to Specify Cell Fates
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Partitioning-defective  Mutants 1) Normally unequal divisions are equal 2) Cleavage spindles are misorientated 3) P granules are mislocalized 4) Blastomere fates are altered These phenotypes imply that partitioning-defective  mutants are lacking gene functions in both spindle  placement and cytoplasmic localization
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Nematodes (Round worms)
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Heterochronic Mutations Mutations that alter the timing of devedlopmental events
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microRNAs or miRNAs
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MicroRNA (MiRNA) Pathway 1) MiRNAs are 19-23 nt long single-stranded RNAs generated  from single-stranded transcript having local-hairpin structure 2) Transcripts are generated by pol II enzyme and possess 5’ cap  and 3’ pol-A tail hallmarks 3) MiRNA maturation begins in the nucleus and terminates in  the cytoplasm 4) The nuclear processing initiates with the endonucleolytic  activity of Drosha, an RNase III enzyme, which in 
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BIOL 595 9-28-09 (F 2009) - BIOL 595 (Fall 2009) 9-28-09...

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