BIOL 595-10-20-09 (F2009)

BIOL 595-10-20-09 (F2009) - BIOL 595 10-20-09 Vertebrate 3:...

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Unformatted text preview: BIOL 595 10-20-09 Vertebrate 3: Neural Induction and Patterning Four Signal Model for Mesoderm formation Neural Induction 04_15a Complete secondary body axis and central nervous system Dorsal Lip Transplant Experiment Relationship of Activin-like Kinases 3/6 and 8 Translation-blocking Morpholinos Expansion of Dorsal Cell Fates Accompanied by a Reduction of Ventral Cell fates Analysis of Morphants by Whole-mount in situ Hybridization Dramatic reduction in phosphorylated (P)- Smad1/5 levels of Alk 3/6 morphants Reduction of P-Smad 1/5 level is similar to that of bmp7-null mutant Reduced levels of P-Smad 1/5 and dorsalized phenotypes of Alk3/6 morphants can be rescued by so-injection of mRNA encoding human ALK3 or mouse Alk6, but not mRNA of Alk8 Alk3a and Alk6a/b function redundant to Alk3b in dorsoventral pattern and combined reduction of alk3 and alk6 gene function abrogates all Bmp signalling during gastrulation. Knockdown Phenotypes Alk3b knockdown did not enhance the class 2 dorsalized phenotype of alk zygotic mutants Loss of alk8 did not enhance the class 3 dorsalized phenotypes of alk3b morphants Alk3a, alk6a,or alk6b knockdown did not further dorsalized alk8 mutants (data not shown) Injection of wild-type alk3a mRNA at amounts that resuce alk3/6 morphants failed to rescue alk8 morphants (Figure 2g or Supp Fig4b) Injection of wild-type alk8 mRNA did not rescue the loss of alk3/6 when supplied in any amount, including at quantities that rescue alk8 mutants (Figure 2g and Supp Fig 4c) Alk3/6 and Alk8 fulfill mutually exclusive roles, functioning non-redundantly in dorsoventral patterning. Heterodimer (bmp2/bmp7)versus Homodimer (bmp2/bmp2 and bmp7/bmp7) Experimental Design:...
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BIOL 595-10-20-09 (F2009) - BIOL 595 10-20-09 Vertebrate 3:...

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