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Lim Development Lecture(III) 11-5-09 F2009

Lim Development Lecture(III) 11-5-09 F2009 - BIOL 595...

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BIOL 595 11-5-09 Limb Development (III)
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Summary Patterning of the limb bud along the proximal- distal axis I) Classical Model : Progress Zone I) Alternative Model: Cell Death and Proliferation
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Figure 1 Cell Death (Apoptosis) and Cell Proliferation To account for the results of AER removal at various developmental stages
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Part 1 of Figure 2 DiI Labeling of Future Stylopod Progenitor Cells Majority of DiI labeled cells die before the formation of the stylopod
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Part II of Figure 2 The ability of Fgf4 to rescue the development of distal elements Is: 1) dependent on the time of Fgf4 addition 2) developmental stage- dependent Rescue by the implantation of Fgf4 soaked beads
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DiI labeled groups of neighbouring cells within a region less than 33uM in diameter and then assayed after 3-4 days later
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Injection of 200- 300 uM below the AER –marked the stylopod (future humerus) Figure 3a Injection of 100- 200 uM below the AER –marked the zeugoopod (future radius/ulna) Figure 3b Injection of directly below the AER –marked the autopod (future digits) Figure 3c DiI Labeling Sub-regions of the Mesenchymal Core Underneath the AER
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