mcb104 handout week 10 - What are the various ways that...

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What are the various ways that mutations arise? Errors in DNA replication Error in meiotic recombination Transposons- unstable genetic elements Chemical and physical assault from the environment Mutagen : chemical or physical agents that increase the rate of mutation above background Ames Test quantitatively tests a chemical’s ability to act as a mutagen. Treat bacteria with the potential mutagen and indirectly measure the mutation rate at specific genetic loci by looking for the ability to grow (the untreated bacteria are unable to grow under the experimental conditions). The bacteria used in this test are auxotrophic for histidine (meaning that they cannot grow in the absence of histidine). The bacteria were specifically designed to have either frameshift or point mutations in genes required for histidine sythnesis. When the bacteria gain the ability to grow in the absence of histidine, it means that they acquired mutations that reverted the mutated genes in the original strain back to functional genes. The bacterial strain also carries mutations in the DNA repair pathway, crippling its ability
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This note was uploaded on 01/28/2010 for the course MCB 104 taught by Professor Urnov during the Fall '09 term at University of California, Berkeley.

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mcb104 handout week 10 - What are the various ways that...

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