mcb104 handout week 5

mcb104 handout week 5 - Lecture 12 Bacterial Genetics...

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Lecture 12: Bacterial Genetics Conjugation is the bacterial version of mating. It is the unidirectional exchange of genetic information, in which DNA is passed from one bacterial cell to another. Lederberg experiment: Poof that bacteria exchange genetic information He used two bacterial strains that were auxotrophic for different nutrients. One strain required thiamine and leucine to live, but had the genes necessary to survive in the absence of biotine and cysteine (i.e. they had genes encoding functional enzymes that were able to synthesize these compounds). The other strain required biotine and cysteine to survive, but had the genes necessary to live in the absence of thiamine and leucine. Niether strain had the ability to grow on minimal media, because they both had specific nutrient requirements. He then mixed the two strains together, and the resulting bacteria had a phenotype distinct from either of the two bacterial strains that he started with. The bacteria that resulted from the mating had
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