Bio1AL_Fa09_lab3_prelab

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Unformatted text preview: of
bacteria.
 
 
 
 
 
 2)

Fill
in
the
following
blanks.

Be
specific
for
the
experimental
conditions
for
Part
1
of
the
enzyme
lab.
 Substrate
(_____)
+
Enzyme
(_____)

reacts
for
____
minutes.

The
reaction
is
stopped
by
the
addition
of
____.

Upon
 heating
reducing
units
react
with
the
added
_______
to
produce
a
brown
soluble
product.

The
absorbance
of
this
 solution
can
be
determined
using
a
spectrophotometer.

A
1µM
maltose
solution
has
an
Optical
Density
of
0.4.

 Therefore
a
solution
with
an
O.
D.
of
0.6
would
represent
a
_____
µM
Maltose
solution.
 
 3)

What
results
would
you
predict
if
you
added
DNS
to
your
reaction
tubes
(after
5
minutes)
and
then
measured
 the
O.D.
of
the
sample
without
heating
(at
or
greater
than
95˚C)?

Explain!
 
 
 
 
 
 4)

Outline
the
general
procedure
to
measure
the
optical
density
of
a
sample.

Include
zeroing
the
transmittance
 (on/off
switch)
and
the
absorbance
(with
the
blank)
of
the
spectrophotometer.

A
diagram
of
the
 spectrophotometer
may
be
helpful.


 
 
 
 
 
 
 
 5)

You
are
given
a
tube
of
spit
that
has
already
been
centrifuged.

Outline
how
y...
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This note was uploaded on 01/28/2010 for the course BIO biology taught by Professor Meighan during the Fall '09 term at University of California, Berkeley.

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