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Bio1AL_Fa09_lab3_prelab

Bio1AL_Fa09_lab3_prelab -...

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A15 – Fall 2009 Transformation and Enzyme Pre‐lab. You must show this completed pre‐lab to your GSI at the start of lab! (Pages 64-90 [focus on 69-76], 91-110 [focus on 91-97) It is due at the end of lab. Thus you can use your flow chart during lab. Name GSI & Sect # Station # To answer these questions read all of the lab material on constructing recombinant DNA (pages 64‐90) even though you are only doing pages 69‐76 for lab 3. You also need to read about enzymes (pages 91‐110). Write legibly. You will NOT be admitted to lab unless you turn in this completed pre‐lab at the start! 1) Define transformation, competent bacteria, ampicillin resistance and “lawn” of bacteria. 2) Fill in the following blanks. Be specific for the experimental conditions for Part 1 of the enzyme lab. Substrate (_____) + Enzyme (_____) reacts for ____ minutes. The reaction is stopped by the addition of ____. Upon heating reducing units react with the added _______ to produce a brown soluble product. The absorbance of this solution can be determined using a spectrophotometer. A 1 μ M maltose solution has an Optical Density of 0.4.
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