Bio1AL_Fa09_lab4_prelab -...

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Unformatted text preview: Picking
a
colony,
Complementation
&
Photosynthesis
Pre‐lab.

 
 Name

 
 
 
 
 

GSI
&
Sect
#

 
 
 
 
 
Station
#
 
 
 To
answer
these
questions
read
all
of
the
lab
material
on
constructing
recombinant
DNA
(pages
64‐90)
even
 though
you
are
only
doing
pages
77
&
78
for
lab
4.

You
also
need
to
read
about
complementation
(pages
111‐ 118)
even
though
you
are
only
doing
pages
111‐113.

You
also
need
to
read
about
photosynthesis
on
pages
119‐ 133.

Write
legibly.

You
will
NOT
be
admitted
to
lab
unless
you
turn
in
this
completed
pre‐lab
at
the
start!
 
 1.

What
color
of
colony
should
you
select
on
your
LB/AMP/X‐gal
plate
if
you
had
an
insert?


 What
color
of
colony
should
you
select
on
your
LB/AMP/X‐gal
plate
if
you
had
no
insert?
 
 
 2.

What
is
histidine?

What
is
the
ploidy
of
the
mutant
yeast
cells
that
you
are
using
in
the
complementation
 experiment?

Are
they
auxotrophs,
or
prototrophs?

Explain
your
answer.


 
 
 
 
 
 
 3.

In
your
own
words,
very
briefly
describe
how
one
performs
pair‐wise
mating
of
yeast
strains.
 
 
 
 
 
 
 4.

What
is
a
complementation
group?
 
 
 
 
 5.

State
the
color/or
absence
of
color
of
the
following
solutions.
 
 a)

a
suspension
of
concentrated
chloroplasts
 
 
 b)

a
1M
solution
of
DCPIP
 c)

a
1M
solution
of
DCPIPH2
 
 
 6.

What
is
an
absorption
spectrum?


How
would
you
prepare
an
absorption
spectrum
for
a
purified
solution
of
 Beta
carotene
dissolved
n
acetone.

You
have
all
of
the
materials
and
equipment
as
outlined
in
the
lab
manual.
 
 
 
 
 
 
 
 7.

In
this
experiment
you
are
measuring
photosynthesis
by
examining
electron
transfer
(reduction
of
DCPIP).

 List
two
other
parameters
you
could
measure
as
indicators
of
rates
of
photosynthesis
(the
experimental
design
 can
be
different).
 
 
 
 
 
 Pre­lab
continued
on
the
backside.
 A21
–
Fall
2009
 8.

In
lab,
you
will
prepare
five
treatments
for
the
Hill
reaction.
 a)

What
is
the
final
concentration
of
the
standardized
chloroplast
suspension
that
you
will
use
in
the
Hill
 reactions?
 
 
 b)

The
final
step
in
preparing
a
standardized
0.1
mg/ml
chlorophyll
solution
is
to
add
“X”
amount
of
your
 enriched
chloroplast
suspension
to
5
ml
of
cold
sucrose
phosphate
buffer.

Therefore
you
must
determine
 how
much
of
your
concentrated
chloroplast
solution
(p.
110,
step
5i)
that
you
need
to
add.

Fill
in
the
 following
table
for
the
given
O.D.
values.

 Final
Volume
(Vf)
 O.D.
Value
 X
=
Volume
of
enriched
chloroplast
suspension
to
5
ml
of
 cold
sucrose
phosphate
buffer
 1.0
 
 
 0.6
 
 
 0.4
 
 
 
 
 c)

How
would
residual
DCMU
(as
a
result
of
failing
to
wash
&
rinse
out
your
test
tubes)
affect
rates
of
 photosynthesis
in
your
light
reactions?
 
 A22
–
Fall
2009
 ...
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