Bio1AL_Fa09_lab5_wksht -...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: GMB
I

Worksheet.

Due
at
the
start
of
your
next
lab.
 
 Name

 
 
 
 
 

GSI
&
Sect
#

 
 
 
 
 
Station
#
 
 
 
 1)

In
lab
you
purified
plasmid
DNA
from
an
overnight
culture
of
bacteria.

What
was
the
number
of
your
culture?
 
 
 2)

Using
your
onion
tip
squash,
draw
and
identify
the
following
stages
of
the
cell
cycle:
interphase,
prophase,
 metaphase,
anaphase
and
telophase.
 
 
 
 
 
 
 
 
 
 
 
 
 3)

Using
the
demonstration
microscope
please
draw
a
cell
in
prophase
I
of
meiosis
and
label
crossing
over.
 
 
 
 
 
 
 
 4)

In
the
following
diagram
of
yeast
matings
indicate
where
a
diploid
 condition
exists
in
a
given
mating
(4
matings
are
shown
with
the
 direction
of
streaking
indicated
with
an
arrow).
 
 
 5)

Are
yeast
cells
prokaryotic
or
eukaryotic?

Are
Escherichia
coli
cells
prokaryotic
or
eukaryotic?

Which
might
 be
best
for
studying
mammalian
genes?
 
 
 
 6)
DNA
Sequencing:
Read
the
sequence
of
the
gel
(of
the
photocopy)
and
write
below.
Indicate
5’
and
3’
ends.
 
 Provide
the
25
nucleotide
sequence
present
in
the
copy
of
the
autoradiogram
that
you
analyzed.
 # 
 1
 2
 3
 4
 5
 6
 7
 8
 9
 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 0
 1
 2
 3
 4
 5
 6
 7
 8
 9
 0
 1
 2
 3
 4
 5
 Nt
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 Which
end
represents
the
5'
end
of
the
sequence?

Explain
your
answer.
 
 A27
–
Fall
2009
 
 
 
 GMB
I
Notes
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 A28
–
Fall
2009
 ...
View Full Document

Ask a homework question - tutors are online