Bio1AL_Fa09_lab6_prelab -...

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Unformatted text preview: DNA
electrophoresis,
Complementation,
and
GMB
II
Pre‐lab.

Due
at
the
start
of
lab.
 
 Name

 
 
 
 
 

GSI
&
Sect
#

 
 
 
 
 
Station
#
 
 
 To
answer
these
questions
read
all
of
the
lab
material
on
constructing
recombinant
DNA
(pages
64‐90)
even
 though
you
are
only
doing
pages
85‐88
for
lab
6.

You
also
need
to
read
about
complementation
(pages
111‐118)
 even
though
you
are
only
doing
pages
116‐118.

You
also
need
to
read
about
GMB
II
(pages
145‐158).

Write
 legibly.

You
will
NOT
be
admitted
to
lab
unless
you
turn
in
this
completed
pre‐lab
at
the
start!

 
 Please
write
your
answers
neatly
&
legibly.

NOTE
WELL:

You
will
not
be
admitted
to
your
lab
unless
you
turn
 in
this
completed
pre‐lab
at
the
beginning
of
your
lab!

The
genetics
portion
is
typically
very
difficult
for
many
 students.

Your
instructor(s)
will
review
the
material
in
lab
and
lecture.
 
 
 1)

Define
the
following
terms:
 
 autosomal
chromosomes
 
 
 
 sex
chromosomes
 
 
 
 genetically
linked
genes
 
 
 
 X‐linked
genes
 
 
 
 
 
 2)

Name
two
purposes
of
the
loading
dye?
 
 
 
 3)

Migration
of
DNA
in
an
agarose
gel
varies
depending
upon
the
size
and
conformation
of
the
DNA.

A
mixture
of
 three
DNA
conformations,
all
having
a
size
of
4,500
base
pairs,
was
loaded
into
a
well
and
the
sample
was
 electrophoresed.

The
banding
pattern
of
the
DNA
is
shown
below.

Note
that
the
band
that
corresponds
to
linear
 DNA
is
labeled.

Indicate
which
of
the
other
two
bands
correspond
to
the
supercoiled
and
the
relaxed
(nicked)
 DNA.
 a)
supercoiled
DNA,
size
4,500
base
pairs
 b)
linear
DNA,
size
=
4,500
base
pairs
 c)
relaxed
(nicked)
DNA,
size
=
4,500
base
pairs
 
 
 Linear
DNA
 
 
 
 
 
 
 4)

What
is
the
phenotype
and
sexes
of
the
flies
used
in
the
Parental
cross?
 
 
 
 Pre­lab
continued
on
the
other
side.
 A31
–
Fall
2009
 Direction
of
 Migration
 
 5)

Write
the
genotype
for
the
following
two
fruit
flies
involved
in
a
cross.

Use
proper
genetic
notation.

The
 female
fly
is
heterozygous
for
dumpy
antennae,
a
recessive
trait
and
homozygous
for
knob
foot,
a
dominant
trait
 (note:
the
wild
type
alleles
for
antennae
and
foot
are
normal
antennae
shape
and
normal
foot
shape).

The
male
 fly
is
heterozygous
for
dumpy
antennae
and
hemizygous
for
normal
foot.

The
dumpy
antennae
locus
is
located
 on
chromosome
#
3
(an
autosome)
and
the
knob
foot
locus
is
located
on
the
X
chromosome
(a
sex
chromosome).


 
 Use
the
letters
da
for
dumpy
antennae
and
kf
for
knob
foot.

You
must
determine
if
they
should
be
upper
 or
lower
case.

To
determine
this
you
must
determine
if
the
mutation
is
recessive
or
dominant?

Complete
a
 Punnett
square
for
the
cross
between
the
female
and
male.

You
must
include
the
genotype
of
the
 offspring.
 
 Genetic
Notation
for
:

male:
 
 
 
 female:
 
 Female
gametes
(across
the
axis,
in
bold)
 Male Gametes 
 6)

In
lab
you
will
examine
2
phenotypes‐‐‐eye
color
and
bristle
shape.

Fruit
flies
will
have
either
red
or
white
 eyes
(alternative
forms
of
eye
color),
and
straight
or
singed
bristles
(alternative
forms
of
bristle
shape).

Assume,
 for
purposes
of
this
worksheet,
the
mutations,
white
eye

and
singed
bristles
are
recessive
(mutations
need
not
 be
recessive).


 Notation:

w
=
white
eyes,
w+
=
red
eyes;
)
sn
=
singed
bristles,
sn+
=
straight
bristles:
(note
use
of
lower
case,
 superscript
with
+
to
indicate
wild
type
 
 These
alleles
are
distributed
on
chromosomes.

Keep
in
mind
that
there
are
two
types
of
chromosomes:
 autosomal
and
sex
chromosomes.

For
the
following
cross
please
show,
with
pictures
and
notation,
the
5
possible
 ways
these
alleles
could
be
distributed
on
chromosomes.

Do
not
place
the
alleles
on
the
Y
chromosome
–
only
X
 or
autosomal.

The
female
came
from
a
pure
breeding
stock
with
white
eyes,
singed
bristles.

The
male
came
from
 a
true
breeding
stock
with
red
eyes,
straight
bristles.

Construct
Punnett
squares
for
each,
and
make
a
prediction
 about
the
F1
for
each.

You
should
indicate
phenotype
and
the
ratio
for
each
type
of
offspring.

Use
the
proper
 notation.

One
possibility
has
been
illustrated.

In
this
case
the
alleles
are
on
different
autosomes‐
one
is
drawn
as
 a
circle,
the
other
as
a
rectangle.

Clearly
the
two
genetic
loci
are
genetically
unlinked
because
they
are
on
 different
chromosomes.

There
are
four
more
possibilities—i.e.

other
than
both
traits
on
different
autosomes.

 (Do
not
place
the
loci
on
the
same
autosome
but
so
far
apart
that
they
appear
to
be
genetically
unlinked
because
 that
was
already
done
for
you.)

Consider
which
locus
you
put
on
which
type
of
chromosome
and
also
how
far
 apart
those
loci
are.

One
possibility
is
shown
on
the
next
page.

You
need
to
indicate
four
other
possibilities.


 
 
 A32
–
Fall
2009
 NOTATION
IS
CRITICAL.

Make
the
connection
between
your
drawing
of
the
chromosomes
with
the
loci
 indicated
and
how
this
is
reflected
in
notation.
 
 
 Female
 
 
 
 Male
 
 
 Punnett
square
 1) w w = w ; sn w sn w+ w+ = w+ ; sn+ w+ sn+ male gametes w+, sn+ = w+ ; sn+ w sn all flies red eyes straight bristles sn sn sn+ sn+ w; sn female gametes Autosomally unlinked 
 2)
 
 
 
 
 
 
 
 
 3)
 
 
 
 
 
 
 
 
 4)
 
 
 
 
 
 
 
 
 5)
 
 
 
 
 
 
 
 
 
 A33
–
Fall
2009
 
 
 
 
 Genetics
and
Molecular
Biology
II
Notes
 A34
–
Fall
2009
 ...
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