Bio1AL_Fa09_lab6_wksht -...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: GMB
II
Worksheet.

Due
at
the
start
of
your
next
lab.
 
 Name

 
 
 
 
 

GSI
&
Sect
#

 
 
 
 
 
Station
#
 
 
 1)

What
were
the
results
of
your
complementation
test?

What
general
conclusions
could
be
drawn
by
 combining
the
data
of
all
groups
in
your
lab?
 
 
 
 
 
 
 2)
Tabulate
your
labs
data
for
the
F2
flies.

Due
to
the
actual
cross
that
was
done
it
is
not
necessary
to
distinguish
 between
the
male
&
female
flies.
 
 Parental
types
 Recombinant
types

 Male
&
female
flies
combined
 
 
 
 
 3

Draw
a
chromosome
containing
the
bristle
genes
and
eye
color
including
map
distance
by
using
your
entire
 labs
data
(from
number
2)
to
calculate
the
map
distance.

NOTE:

%
of
recombinants
is
determined
by
taking
the
 number
of
recombinants
divided
by
the
total
#
of
progeny
that
can
show
recombination
in
that
particular
cross!!

 Sometimes
recombination
may
occur
but
it
will
be
masked
by
dominant
alleles
and
hence
those
offspring
cannot
 display
the
recombinant
phenotype.
 
 
 %
recombination
=



_______
 
 
 
 
 
 
 
 4)

A
female
fruit
fly
heterozygous
for
stubby
antennae
and
clipped
wings
(wings
normally
are
long),
both
 recessive
alleles,
was
mated
to
a
true‐breeding
wild
type
male.

The
F1
offspring
were:
 

 
 
 





males
 




females
 stubby
and
clipped
 34
 0
 stubby
and
long
 564
 0
 normal
and
clipped
 566
 0
 normal
and
long
 36
 1000
 
 A.


Please
construct
a
Punnett
square
that
demonstrates
why
all
of
the
females
are
of
the
wild
type.


 
 
 
 
 
 
 B.


Draw
the
mother's
genotype
in
genetic
notation.

Please
double
check
to
see
that
your
answer
is
correct
(i.e.
 does
it
agree
with
the
data).
 
 
 
 
 C.


If
the
genes
are
linked,
how
far
apart
are
they?

Show
your
work.



 
 
 Worksheet
continued
on
the
other
side.
 A35
–
Fall
2009
 5)

A
mutant
fruit
fly
was
found
with
a
pearl
eye
color
and
twisted
wing.

When
a
female
from
a
population
of
 true‐breeding
pearl‐eyed
and
twisted
wing
flies
was
mated
to
a

male
from
a
true‐breeding
wild
type
population,
 all
 of
 the
 F1
 progeny
 were
 pearl‐eyed
 with
 wild
 type
 wings.
 
 What
 information
 does
 this
 cross
 tell
 us?
 
 What
 information
 does
 it
 not
 tell
 us?
 
 To
 answer
 this
 question
 construct
 4
 Punnett
 squares
 for
 the
 various
 possible
 ways
that
this
genetic
information
can
be
contained
in
the
fruit
flies.
 
 a)
both
traits
autosomal,
not
linked
 b)
one
trait
on
an
X
chromosome,
the
other
on
an
autosomal
chromosome
 c)
both
traits
linked
on
an
autosome
 d)
both
traits
linked
on
an
X
chromosome
 
 Use
this
page
to
show
your
reasoning.
 A36
–
Fall
2009
 
 6)

Generate
your
standard
curve
(HinD
III
digest
of
λ
DNA)
on
the
semi‐log
graph
paper
on
the
back.


 
 
 7a)

Indicate
the
number
of
your
tube
used
for
digestion.

 
 
 7b)

Indicate
the
size
of
the
various
fragments
produced
when
digesting
your
plasmid
DNA
(number
1‐14).
 
 
 
 8)

Your
GSI
or
UGSI
loaded
two
DNA
samples,
lanes
8
&
9.

How
many
bands
are
present
in
each
lane
and
how
 far
did
each
band
migrate?

Determine
the
base
pair
length
of
DNA
fragments
found
in
each
band.


 lane
8
(cut
pBLU):
 lane
9
(supercoiled):
 
 
 
 9)

Indicate
the
size
of
the
various
fragments
produced
when
digesting λ DNA
with
the
restriction
enzyme
Eco
 R1.

(Note
that
there
may
not
be
five
bands)
 i)

 
 ii)

 
 iii)

 
 iv)

 
 v)

 
 
 A37
–
Fall
2009
 Semi‐
log
paper

 (Plot
Base‐pair
size
(y‐
axis)
vs.
distance
migrated
(x‐axis)
 
 A38
–
Fall
2009
 ...
View Full Document

Ask a homework question - tutors are online