{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Final09WhiteKey - Name White Key Last First BIS102-02...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Name White Key BIS102-02, Spring '09, Page 1 of 11 Last, First C. S. Gasser BioSci 102-02 June 6, 2009, Final Exam Instructions: There are eleven pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. For calculation problems a clear line of calculations leading to your final answer must be shown to obtain full credit. Use the following pK a values for the exam. Amino Acid α -NH 2 α -COOH R-group log 1.0 = 0.00 Ala, Gly, Ile, Leu, Val 9.7 2.3 log 1.5 = 0.18 Asn, Gln 9.0 2.1 log 2.0 = 0.30 Met, Ser, Trp 9.3 2.3 log 2.5 = 0.40 Phe 9.1 1.8 log 3.0 = 0.48 Pro 10.6 2.0 log 3.5 = 0.54 Thr 10.4 2.6 log 4.0 = 0.60 Asp 9.8 2.1 3.9 log 4.5 = 0.65 Glu 9.7 2.2 4.2 log 5.0 = 0.70 His 9.2 1.8 6.0 log 5.5 = 0.74 Cys 10.8 1.8 8.3 log 6.0 = 0.78 Tyr, Lys 9.1 2.2 10.0 log 6.5 = 0.81 Arg 9.0 2.2 12.5 log 7.0 = 0.85 log 7.5 = 0.88 Question Val 1 ue Score log 8.0 = 0.90 1 30 log 8.5 = 0.93 2 12 log 9.0 = 0.95 3 20 log 9.5 = 0.98 4 18 log 10.0 = 1.00 5 12 6 14 R = 8.3 x 10 -3 kJ/°K•mol 7 20 25°C = 298K 8 21 9 16 10 8 11 6 12 8 13 15 Total 200
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Name White Key BIS102-02, Spring '09, Page 2 of 11 Last, First C. S. Gasser 1. You have been given two samples of blood. One sample comes from a child that is believed to have consumed plant material containing malonate, an inhibitor of succinate dehydrogenase (SDH) an essential metabolic enzyme. The second sample comes from a healthy child. Unfortunately, the samples were only labeled only with the letters “A” and “B” and you don’t know which is which. You go ahead and perform enzyme assays to test the effects of addition of portions of the two samples on SDH activity to try to detect the effects of inhibitor. The results for sample A are plotted below, and the results for sample B are given in the table. Use this information to answer the questions below. 1.0 0.8 0.6 0.4 0.2 0.0 -0.2 -0.4 -0.6 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 1/[S] 1/V A (min • ml/nmole) (1/μM) B Data for Sample B [S] (μM) V (nmole/ml•min) 1/[S] 1/V 2.5 2.0 0.4 0.5 5 3.3 0.2 0.3 10 5.0 0.1 0/2 20 6.7 0.05 0.15 a) (6) Plot the data for sample B on the axes above using the units as labeled. Plot 1/V vs. 1/[S] (calculations at right in table above) b) (4) Which sample appears to be the control sample not containing malonate (circle one)? B has an apparent increase in the Km. So A has no inhibitor. A B
Image of page 2
Name White Key BIS102-02, Spring '09, Page 3 of 11 Last, First C. S. Gasser c) (8) What are the K m and V max for SDH in the assay that does not contain malonate? -1/K m = -0.5, K m = 2 μM 1/V max = 0.1 so V max = 10 nmol/min•ml K m = 2 μM V max = 10 nmol/min•ml units units d) (8) You determine that the concentration of malonate in the inhibited samples is 2 μM. What is the K I of malonate for SDH? -1/K mapp = -0.1, K mapp = 10 μM K mapp = Km(1 + [I]/K I ) 10 = 2(1 + 2/K I ); 5 = 1 + 2/K I ; 4 = 2/K I ; K I = 2/4 = 0.5 μM K I = 0.5 μM units e) (4) The diagram below shows the mechanism of an enzyme catalyzed reaction. Complete the diagram by showing how malonate (I) would interact with SDH (E) and S?
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern