BIS103 Key Final F09

BIS103 Key Final F09 - 1 of3 BIS 103 Name l4 Fall 2009 Last...

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Unformatted text preview: 1 of3 BIS 103 Name l4 Fall, 2009 Last 3 First K. Hilt Final Exam Score (200): Equations: pH = pKa + log {[b]/[a]} A = e l c Constants: 8 of NADH and NADPH at 340 nm = 6220 M'lcm'l 1. (5 pts.) The Calvin cycle has three stages. Give the name of each stage: Stage # Name 1 Qt‘xa-Li M 2 rg & 55+: iM 3 raéwu 4:“ M 2. (10 pts.) One of the reactions in either glycolysis or the Kreb’s cycle involves the addition of a water molecule to a trans double bond. Write the balanced chemical reaction for this step, using structures that predominate at pH 7, and give the name of the enzyme that catalyzes this reaction. A [c170 are“ ‘ c gummx l H _ c-D ll *Y “20 f H- ‘ / ”00C \l" C342 coo“ 3. (10 pts.) Write the balanced chemical reaction for the first committed step in glycolysis. Use structures that predominate at pH 7 and give the name of the enzyme that catalyzes this step. _ 44 CA4 H @ 0 LC 0 20 @0~ Hzc [email protected] {'3 ——————5’ 0 0H 4' A19 hnselflb‘q’fllm I H0 “ -\" AD? 0” 6 kinasfl 0 H 0 4. (70 pts.) Put the name of each molecule on the line that fits each description (each is worth 5 pts.): a) C O 2 coenzyme of rubisco b) P Q ( cufiogfg‘nm) hydrophobic, mobile e' carrier in thylakoid membranes c) 11 201; ad coenzyme in branched-chain a-keto acid dehyhdrogenase that carries electrons and branched-chain molecules d) b‘m Mex coenzyme that carries C02 in gluconeogenesis e) G T P ( err?) nucleotide triphosphate produced in the Kreb’s cycle 1) ubf — at“: 5:. nucleotide diphosphate compound that is involved in glycogen biosynthesis ’ g) e)g 5+0 cgam'm mobile one e' carrier protein that operates between phostosystem I and 11 h) /l f , m‘ bulose begeltosglwle acceptor molecule in rubisco catalyzed reaction 1) u .‘ — 5 - Co A 4~carbon thioester in the Kreb’s cycle j) agei‘od Aehgde 2-carbon aldehyde in alcoholic fermentation k) D H A P compound that when it exits the chloroplast, allows simultaneous entrance of P] l) 6,33: E! g functional equivalent of complex III in ETC, but in chloroplasts m cobra-\tne. w lw‘l’an absorbs a photon of light in plants, but has no Mg+2 ion ETC complex that accepts e"s from succinatc of Kreb’s cycle 20f3 BIS 103 Name [(519 5. (15 pts.) The energy diagram below refers to something that we discussed in class. You need to do three things: 1) identify what is being discussed, 2) explain briefly why this energy diagram is the way it is, and 3) identify the cellular processes that have this in them (that we discussed in class). #1.Whatisbeingdiscussed? ATP sign-“644's 51’ ATP 5,..an #2 Wh isthisdiagramthiswa 9 Lcu nmlm wen}— bub haw m Jaws-«1% Wait M15- IM Hair mag \15. Reaction Coordinate #3. Cellular processes that this 18 part of: ETC mg Phohsjn‘lfim 6. One of the enzymatic reactions in the Kreb’s cycle involves a phosphorylated histidine at the enzyme site, as part of the reaction mechanism. (5 pts.) a) Draw the phosphorylated histidine side chain as it exists in the enzyme active site: g- F»?! anced chemical reaction of this Kreb’s cycle reaction, in the form that would 0:4 er gm"?! Cafl’ SMQaW K Linhfi» 5Mcih¢~CoA 4' GDP 4- YL——————-§ 6T? * swam, +— QA5H (10 pts.) b) Write Ii - predominate at pH 7. Give the enzyme name (5 pts.) 0) Why was the phosphorylated histidine in part “a” above important in the reaction mechanism? wt) (Mathilda glow». W 7. Photosynthesis in plants has a “3:2 ratio” of two difi'erent compounds. (5 pts.) a) What are these two compounds? Which is “3” and which is “2”? 3 AT? ‘- 2 :4 ANN (10pts)b)WhereintheZ—schemeiseachproduced?Beveryspecific. :3 ATP is "W 5 ATP mamma— wkl‘o“ run) It“ 4 m #* 7rd“ emu b4? aybze and. m 051: wlq A NM?“ .5 mask. in, #errg‘Lum "App‘l’ “54"de .f-Ha. coal if, 4417540”; (10 pts. ) c) Where' in the Calvin cycle 18 each consumed? Be very specific. I) PH? \5 0“st M We. rfxuhm “A rejaer‘ahm PM 3"“ _-, L3 996' (Amine—SQ —-v I :- ribulm bisp‘wée 2) NPFDPH X6 Wank 1.; 1“. WM ska-u. 1,33% —» as? 3of3 BIS 103 Name £11} 8. In class, we discussed how we could run a coupled enzyme assay to measure the enzyme activity of acetate kinase. (5 pts.) a) Write the balanced chemical reaction catalyzed by this enzyme, starting with acetate. Use chemical structures for acetate and aeetylphosphate, in the forms that would predominate at pH 7 (write out phosphate; do m; use a circled P). You do not need to draw the structures of ADP or ATP. 0 O o | t‘ - OH3'IL-D’ ‘l’ A179 "#9 CH3— L'O'P‘ro 4. A)? 0 (15 pts.) b) Write out the coupled enzyme reactions that together measure the AA34o/Atime of NADH being consumed. Your coupled reactions should start with your reaction in part “a” above and include the glycolytic reactions catalyzed by lactate dehydrogenase and pyruvate kinase, in the necessary order. Each chemical reaction should be balanced and include chemical structures that predominate at pH 7 (except for things like NAD+, ADP, etc.). Put a check mark ( V ) next to every item in your coupled assay that should not be rate limiting. / / a P CH3 can“ 4r PrTP i ofi3~'c—~n—® + Nb? / PE C 07’ / L - 0 —® “‘2 ~ ‘/ if pgruvafiv 1'3an 009 ‘fwvm ‘ obi ./ l’ + 3/ LDH U,- NABH ++l+g:_,‘—>|M-L H + “rib." Lila \¢L+'bhi¢r (10 pts.) c) Your coupled enzyme assay in part “b” works! Your 3 ml assay, which has everything in it that you discussed in part “b” above, gives a AAm/A 5 min. = -0.358/5 min. How many I.U.’s of acetate kinase were in your 3 ml assay? Put your answer here: _Q:__0_3_‘i§___ I. U ’s. To receive credit, show all of your work below. Aflw : e ,0 Ac. 9.0714: 2 522,, H m '0 ug( ozuabfl) y I. ISx/Dhsl'f = ADM-lb?” 0% : 0.0'7I/o/M M, -3 04/ . <l.l§’~ ”~524— )(o,aa31) = 3.55'110 M 1% M 2 0.034? 1.11- 9. (15 pts.) Explain what is known about the mechanism of water splitting in photosystem II of plants. Begin your detailed discussion with “An exciton. . .” End your discussion with a brief statement of what is notknown. + l. pm WCA‘LIIW +¢M5<w {-0 p690 Creeks P680 . 2, 9LSD+ Joel-racks an e." gown“ firming Z, WMJA Hm M 9: "9"“ m Mm“ Wlw. A H4" «‘5 released. {A1 “4.2, lam. 3, fly-0cm i7. 0M5 Ll 4m“ 4, 2 H20 from“ M “5““3 Ll e,' 5 +0 "lira. Mn.” “'1'"! M m L-\ ‘P 4—, Ha lam _; H awe. abs+vac+c¢9 W Hlo W “N” ". 1+ I5 1104 known hm +91 b M“. 9,"; are. Ava/“slur.“ and-o ~Haq. lam. ...
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