BIS102 - MT1_Sp09

BIS102 - MT1_Sp09 - Name Green Key Last, First BIS102-02,...

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Name Green Key BIS102-02, Spring '09, Page 1 of 7 Last, First C. S. Gasser BioSci 102-02 First Midterm, Apr. 21, 2009 Instructions: There are seven pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. For calculation problems a clear line of calculations leading to your final answer must be shown to obtain full credit. Use the following pK a values for the exam. Amino Acid α -NH 2 α -COOH R-group log 1.0 = 0.00 Ala, Gly, Ile, Leu, Val 9.7 2.3 log 1.5 = 0.18 Asn, Gln 9.0 2.1 log 2.0 = 0.30 Met, Ser, Trp 9.3 2.3 log 2.5 = 0.40 Phe 9.1 1.8 log 3.0 = 0.48 Pro 10.6 2.0 log 3.5 = 0.54 Thr 10.4 2.6 log 4.0 = 0.60 Asp 9.8 2.1 3.9 log 4.5 = 0.65 Glu 9.7 2.2 4.2 log 5.0 = 0.70 His 9.2 1.8 6.0 log 5.5 = 0.74 Cys 10.8 1.8 8.3 log 6.0 = 0.78 Tyr, Lys 9.1 2.2 10.0 log 6.5 = 0.81 Arg 9.0 2.2 12.5 log 7.0 = 0.85 log 7.5 = 0.88 Question Value Score log 8.0 = 0.90 1 23 log 8.5 = 0.93 2 15 log 9.0 = 0.95 3 24 log 9.5 = 0.98 4 12 log 10.0 = 1.00 5 18 6 16 R = 8.3 X 10 -3 kJ/°K•mol 7 12 25°C = 298K 8 30 Total 150
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Name Green Key BIS102-02, Spring '09, Page 2 of 7 Last, First C. S. Gasser 1. (23) You are analyzing a peptide isolated from cancer cells. The peptide consists of six amino acid residues. Partway through amino acid analysis the computer running the amino acid analyzer crashes preventing you from determining all of the amino acids. You are able to determine that the peptide contains the following residues: Asp, Ile, Lys, Tyr, Thr, and one other undetermined amino acid. From this and the following information determine the sequence of the peptide. Briefly list conclusions that can be drawn from each piece of information provided. a) (4) When treated with fluorodinitrobenzene (FDNB) followed by complete hydrolysis to
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BIS102 - MT1_Sp09 - Name Green Key Last, First BIS102-02,...

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