Introduction to Enzymes - BIOC 460 Sample Problems on...

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Sample Problems on Introduction to Enzymes 1. Assume an uncatalyzed reaction. Suppose that k F = 5 x 10 –4 s –1 , and K eq = 2.5 x 10 2 . What is k R ? k R(uncat) = k F(uncat ) /K eq(uncat) = 2 x 10 –6 s –1 2. Consider the same enzyme as in #1, with rate constants and equilibrium constant given or calculated in #1 for the uncatalyzed reaction. Suppose that in the presence of an enzyme, k F(catalyzed) = 5 x 10 5 s –1 A. What is the rate enhancement brought about by the enzyme? rate enhancement = k (cat) / k (uncat) k F(cat) / k F(uncat) = 5 x 10 5 s –1 / 5 x 10 –4 s –1 = 1 x 10 9 (Rate enhancement is always unitless) B. What is k R(catalyzed) ? Catalyst must increase k R by the same factor that it increased k F k R(uncat) (calculated in #1) = 2 x 10 –6 s –1 Rate enhancement = 10 9 = k R(cat) / k R(uncat) so k R(cat) = 10 9 x k R(uncat) = 2 x 10 3 s –1 C. What is K eq for the catalyzed reaction? Catalyst doesn
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This note was uploaded on 01/30/2010 for the course BIOC 460 taught by Professor Ziegler during the Spring '07 term at University of Arizona- Tucson.

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Introduction to Enzymes - BIOC 460 Sample Problems on...

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