practice midterm 2 key

practice midterm 2 key - (Multiple choice problems are...

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(Multiple choice problems are worth 3 points each). 1) Cyclin-dependent kinase (CDK) regulates replication initiation in eukaryotes by: a) preventing assembly of pre-replicative complexes during S, G2, and M phase b) activating pre-replicative complexes when cells enter S phase c) phosphorylating replication proteins d) all of the above e) none of the above 2) The proofreading function of DNA polymerases is: 3) How many telomeres are found in a human sperm or egg? 4) Holliday junction migration is promoted by: 5) With respect to DNA topology, DNA replication causes: a) positive supercoiling ahead of the replication fork b) DNA unwinding at/behind the replication fork c) catenation of DNA ahead of the replicaton fork d) all of the above 6) Making a specific gene deletion in any organism requires: a) transposon mutagenesis b) knowledge of the gene’s function c) a haploid cell d) homologous recombination 7) A diploid yeast cell has a normal and a mutant allele of a gene involved in biosynthesis of histidine. A haploid yeast cell with the mutant allele of this gene is unable to grow without histidine added to its growth medium; however, the diploid cell described above is able to grow without added histidine. This is due to: 8) (4 pts.) Draw a replication fork in the process of DNA replication.
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